TRH & Metoclopramide Test

Purpose
Pituitary stimulation by thyroliberin or TRH and metoclopramide to monitor prolactin induction by TRH and then the effect of a dopamine antagonist on prolactin release.

Subject preparation
Fasting and resting for 30 min.

Product
- TRH (Stimu-TSH®, 250 μg vial).
- Primperan® (10 mg / 2 ml vial).

Dose
- Adults: 250 μg Stimu-TSH®
- Children: 200 μg/m2 body surface area, without exceeding 250 μg
- Adults and children: 10 mg Primperan®.

Protocol
- Samples (dry or EDTA tubes) at time intervals: 15 min (T-15) and 0 (T0).
- Slow IV injection of Stimu-TSH®.
- Samples (dry or EDTA tubes) T+15, T+30 and T+60 min after the injection of TRH.
- After the T+60 sample, administer one vial of Primperan (10 mg) by slow intravenous injection
- Samples (dry or EDTA tubes) T+75, T+90 and T+120 min after the injection of TRH.

Assay
Prolactin.

Side effects
Rare and transient: hot flushes, pins and needles, nausea, headache, dizziness.