Bone Marrow Unit

It's our honor and great pleasure to introduce to you the services available at the bone marrow unit at Saridar lab:

1) Bone marrow aspiration (sternal or iliac puncture):

*Morphological examination.

*Cytochemical staining needed for proper diagnosis: Iron stain, myeloperoxidase / or suddan black, chloracetate esterase, leucocyte alkaline phosphatase score (LAP score), non specific esterases (NASDA/NASDA-F), acid phosphatase and PAS.

*Immunophenotyping by flow cytometric analysis of aspirated bone marrow samples (or peripheral blood sample containing more than 15% malignant cells) for diagnosis and classifications of hematological malignancies.

The markers used are:

(1)  CD45 (Common Leucocytic Antigen)

(2)  B-cell markers: CD22, CD10, CD19, CD20, CD79a, cytoplasmic IgM, kappa and lambda light chain

(3)  T-cell markers: CD3, CD2, CD5, CD7, CD4, CD8 and CD1a

(4)  Myeloid markers: CD13, CD33, MPO, CD117. CD14 and CD15, CD64, CD11c (monocytic markers)

(5)  Megakaryocytic markers CD61, CD42 and CD41

(6)  Stem cell marker: CD34

(7)  Markers for paroxysmal nocturnal haemoglobinuria

(8)  DNA index and cell cycle analysis

(9)  Apoptotic markers: Annexin V and propedium iodide

*Cytogenetic study of aspirated B.M specimen for chromosomal abnormalities: translocation, deletion, inversion, monosomy or trisomy.

2) Bone marrow trephine biopsy:

(1)Sample taken under local anesthesia.

(2)Hamatoxylin and eosin smear examined by both consultants of hematology and pathology.

(3)Immunocytochemical stains needed for proper diagnosis: B-cell markers / T-cell markers / markers for Hodgkin^'s disease (CD15, CD30) / markers for solid malignancy e.g. cytokeratin (CK), S-100 protein, Epithelial Membrane Antigen (EMA).